| Show | Gays in Space |
| Peter Sarsgaard | ... | Captain Trip Bunchkin |
| Fred Armisen | ... | Billiam |
| Rachel Dratch | ... | Loretta |
| Tina Fey | ... | space lesbian |
| Will Forte | ... | singer |
| Bill Hader | ... | host |
| Steve Higgins | ... | announcer Voice |
| Seth Meyers | ... | Pegasus |
| Finesse Mitchell | ... | ex-boyfriend |
| Chris Parnell | ... | Thad |
| Paula Pell | ... | Judine |
| Paula Pell | ... | announcer Voice |
| Jason Sudeikis | ... | Paolo |
| Kenan Thompson | ... | Givindy |